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KMID : 0613820140240111187
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Journal of Life Science
2014 Volume.24 No. 11 p.1187 ~ p.1192
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Analysis of Genes Expressed during Pepper-Phytophthora capsici Interaction using EST Technology
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Kim Dong-Young
Lee Jong-Hwan
Choi Woo-Bong
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Abstract
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Pepper, consumed as a typical spice food around world, is mainly cultivated in warm countries, including Korea, China, and Mexico. Phytophthora capsici is a pathogen on several economically important crops, including pepper. The oomycete attacks the roots, stems, leaves, and fruit of the host plants. To understand the molecular mechanisms underlying development of the disease, the genes expressed during pepper-P. capsici interaction were explored by analyzing expressed sequence tags (ESTs). A cDNA library was constructed from total RNA extracted from pepper leaves challenged with P. capsici for three days, resulting in an early stage of symptom development for comparable interaction. A comprehensive analysis of single-pass sequencing of 5,760 randomly selected cDNA clones extracted 5,148 high-quality entries for contig assembly, which generated 2,990 unigenes. A homology search of the unigenes with BLASTX resulted in 2,409 matches, of which 606 showed classified functional catalogs.
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KEYWORD
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Expressed sequence tag (EST), pepper, Phytophthora capsici
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